Blood and Urine Tests

A Study Guide

Understanding Reference Ranges: A reference range is a set of values that includes 95% of the normal, healthy population. A result outside this range doesn't automatically mean disease, and a result within it doesn't guarantee health. All test results must be interpreted in the context of the individual patient's clinical presentation.

Full Blood Count (FBC)

Red Blood Cells (RBCs)

TestNormal RangeClinical Significance
Haemoglobin (Hb)Men: 130-180 g/L
Women: 115-165 g/L
Measures oxygen-carrying capacity. Decreased in anemia. Increased in polycythemia or dehydration.
Haematocrit (Hct)Men: 40-50%
Women: 36-47%
Percentage of blood volume occupied by RBCs. Parallels Hb levels.
Mean Corpuscular Volume (MCV)80-98 fLAverage size of one RBC. Used to classify anemia as microcytic (<80), normocytic (80-98), or macrocytic (>98).
Mean Corpuscular Haemoglobin (MCH)27-32 pgAverage weight of Hb in each RBC.
Mean Corpuscular Haemoglobin Concentration (MCHC)300-350 g/LConcentration of Hb in RBCs. Used to classify anemia as hypochromic (low) or normochromic (normal).

White Blood Cells (WBCs)

TestNormal RangeClinical Significance
Total WCC4-10 x 10⁹/LMeasures total number of leukocytes. High (leukocytosis) suggests infection/inflammation. Low (leukopenia) suggests bone marrow suppression.
Neutrophils2.5-7.5 x 10⁹/L (40-75%)Increased (neutrophilia) in bacterial infections and tissue damage.
Lymphocytes1.0-3.5 x 10⁹/L (20-45%)Increased (lymphocytosis) in viral infections.
Monocytes0.2-0.8 x 10⁹/L (2-10%)Increased in chronic bacterial infections (e.g., TB) and autoimmune diseases.
Eosinophils0.04-0.4 x 10⁹/L (1-6%)Increased (eosinophilia) in allergic reactions and parasitic infections.
Basophils0.01-0.1 x 10⁹/L (0-1%)Increased in some allergic reactions.

Platelets

TestNormal RangeClinical Significance
Platelet Count150-400 x 10⁹/LMeasures platelets (thrombocytes). Low (thrombocytopenia) increases bleeding risk. High (thrombocytosis) can increase thrombosis risk.

Inflammatory Markers

TestNormal RangeClinical Significance
Erythrocyte Sedimentation Rate (ESR)< 16 mm/hourA non-specific marker of inflammation. Elevated in infections, autoimmune diseases, and malignancy.
C-reactive Protein (CRP)< 5 mg/LA more sensitive and specific marker of acute inflammation. Rises quickly in response to infection and tissue injury.

Iron Studies

TestNormal RangeClinical Significance
Serum Iron10-30 µmol/LMeasures iron bound to transferrin. Highly variable and of little value alone.
Serum Ferritin15-300 µg/LMeasures the body's iron stores. Best indicator of iron deficiency (low levels).
Transferrin / TIBC1.7-3.0 g/L / 45-80 µmol/LTransferrin is the main iron transport protein. Both increase in iron deficiency.
Transferrin Saturation15-45%Percentage of transferrin saturated with iron. Decreased in iron deficiency, increased in iron overload.

Glucose Tests

TestNormal / Desirable RangeClinical Significance
Fasting Blood Glucose3.0-5.5 mmol/L> 7.0 mmol/L is diagnostic for diabetes mellitus.
Random Blood Glucose3.3-7.7 mmol/LUsed for opportunistic screening.
Oral Glucose Tolerance Test (OGTT)2-hour level < 7.7 mmol/LUsed to diagnose diabetes when fasting glucose is borderline. A 2-hour level > 11.1 mmol/L is diagnostic.
Glycosylated Haemoglobin (HbA1c)3.5-6.0% (15-42 mmol/mol)Reflects average blood glucose over ~3 months. Used to monitor long-term control in diabetics (target < 7.0%).

Lipid Profile

TestDesirable LevelClinical Significance
Total Cholesterol (TC)< 5.5 mmol/L (Ideal < 4.0)Total amount of cholesterol. High levels are a risk factor for atherosclerosis.
LDL Cholesterol ("Bad")< 3.0 mmol/LTransports cholesterol to tissues. High levels are a major risk factor for atherosclerosis.
HDL Cholesterol ("Good")> 1.0 mmol/LTransports excess cholesterol back to the liver. High levels are protective.
Triglycerides (TG)< 1.7 mmol/LA type of fat in blood. High levels can be an independent risk factor for atherosclerosis.

Cardiac Markers

MarkerNormal RangeClinical Significance
Cardiac Troponin (cTnT, cTnI)UndetectableThe most sensitive and specific markers for myocardial necrosis (MI). Rise within 3-6 hours of an MI.
Creatine Kinase (CK-MB)Essentially 0% of total CKAn isoenzyme found mainly in the heart. Rises in MI but is less specific than troponins.
MyoglobinVariableAn early but non-specific marker of muscle injury (cardiac or skeletal).

Liver Function Tests (LFTs)

TestNormal RangeClinical Significance
BilirubinVariable (Total < 21 µmol/L)Increased levels cause jaundice. Helps differentiate hepatocellular vs. cholestatic liver disease.
ALT (Alanine Aminotransferase)< 40 IU/LA specific marker for hepatocellular injury (e.g., viral hepatitis, toxic damage).
AST (Aspartate Aminotransferase)< 40 IU/LLess specific than ALT (also in heart/muscle), but a sensitive marker of liver cell damage.
ALP (Alkaline Phosphatase)30-120 IU/LElevated in cholestasis (bile duct obstruction) and certain bone diseases.
GGT (Gamma-Glutamyl Transferase)< 50 IU/LA sensitive marker of liver cell damage and cholestasis. Elevated by alcohol consumption.
Albumin35-50 g/LA true test of liver *synthetic function*. Low levels suggest chronic liver disease or malnutrition.

Tests of Haemostasis

TestNormal / Desirable RangeClinical Significance
Prothrombin Time (PT) / INR11-13 sec / INR ~1.0Measures extrinsic/common pathways. Prolonged in liver disease, vit K deficiency. Used to monitor warfarin (target INR 2-3).
Activated Partial Thromboplastin Time (aPTT)25-35 secMeasures intrinsic/common pathways. Prolonged in haemophilias. Used to monitor heparin.

Kidney Function Tests

TestNormal RangeClinical Significance
Urea3-8 mmol/LWaste product of protein metabolism. Increases with impaired renal function or dehydration.
Creatinine< 0.12 mmol/LWaste product of muscle metabolism. A more reliable indicator of renal function than urea.
eGFR (Estimated GFR)> 90 mL/min/1.73m²Best overall indicator of kidney function. A value < 60 suggests chronic kidney disease.

Electrolytes

TestNormal RangeClinical Significance
Sodium (Na⁺)135-145 mmol/LMajor extracellular cation. Regulates osmotic pressure. Imbalances often reflect water balance issues.
Potassium (K⁺)3.5-5.3 mmol/LMajor intracellular cation. Crucial for nerve and muscle function. Imbalances can cause life-threatening cardiac arrhythmias.
Calcium (Ca²⁺)2.1-2.6 mmol/L (total)Crucial for muscle contraction, nerve function, and blood clotting. Imbalances affect neuromuscular excitability.
Chloride (Cl⁻)96-106 mmol/LMajor extracellular anion. Levels generally follow sodium.

Arterial Blood Gas (ABG) Analysis

ParameterNormal RangeSignificance
pH7.35 - 7.45Measures acidity/alkalinity. < 7.35 = Acidosis, > 7.45 = Alkalosis.
PaCO₂35 - 45 mmHgThe respiratory component. High in respiratory acidosis (hypoventilation), low in respiratory alkalosis (hyperventilation).
HCO₃⁻22 - 26 mmol/LThe metabolic component. Low in metabolic acidosis, high in metabolic alkalosis.
PaO₂80 - 100 mmHgMeasures adequacy of blood oxygenation.
Oxygen Saturation (SaO₂)> 95%Percentage of haemoglobin saturated with oxygen.

Urine Tests (Urinalysis)

TestNormal FindingAbnormal Finding Suggests
Specific Gravity (SG)1.010 - 1.025Hydration status. High SG = dehydration, Low SG = overhydration or diabetes insipidus.
pH4.6 - 8.0Can indicate systemic acid-base disorders or urinary tract infections.
GlucoseNegativeDiabetes mellitus.
KetonesNegativeDiabetic ketoacidosis, starvation.
ProteinNegative/TraceKidney disease (e.g., glomerulonephritis, nephrotic syndrome).
BloodNegativeUTI, kidney stones, glomerulonephritis, malignancy.
LeukocytesNegativeUrinary tract infection (UTI).
NitritesNegativeBacterial UTI (many common bacteria convert nitrates to nitrites).